7. Use
the PCR product sequences from 6., search a suitable restriction enzyme(s).
545 base pairs
gccagtgtatcaactcaactgctgttaaatggcagtctagcagaagaagagataataatcagatctgaaatctca base pairs
cggtcacatagttgagttgacgacaatttaccgtcagatcgtcttcttctctattattagtctagactttagagt 1 to 75
caaacaatgcaaaaccataatagtcactaatgaatctgtagaaataatt#gtacagaccccaacaacaatacaaga base pairs
gtttgttacgttttggtattatcagtgattacttagacatctttattaacatg#tctggggttgttgttatgttct 76 to 150
aaaagtataaatataggaccagggagagcatattctataacaggagacataataggagatataagacaagcatat base pairs
ttttcatatttatatcctggtccctctcgtataagatattgtcctctgtattatcctctatattctgttcgtata 151 to 225
tgtaattatggaacaaaatggaataaactttaaaacaggtagctaaaattaaaagacatttaaaaaaataagaaa base pairs
acattaataccttgttttaccttatttgaaattttgtccatcgattttaattttctgtaaatttttttattcttt 226 to 300
aaaaaacaataatctttaacacctcaggagggaccagaaattcaatgcatttttaattgtgaggggaatttttct base pairs
ttttttgttattagaaattgtggagtcctccctggtctttaagttacgtaaaaattaacactccccttaaaaaga 301 to 375
atgaatacaacacaactgtttaatagtacttggaaaatgaaaaaaaaaaaccagaggggtaatgacacaaaaaaa base pairs
tacttatgttgtgttgacaaattatcatgaaccttttactttttttttttggtctccccattactgtgttttttt 376 to 450
aaaatatcacactccatgcagaataaaacaaattataaacatgtggcaggagtaggaaagcaatgtatgccctcc base pairs
ttttatagtgtgaggtacgtcttattttgtttaatatttgtacaccgtcctcatcctttcgttacatacgggagg 451 to 525
catcagtggaaattaatgtt base pairs
gtagtcacctttaattacaa 526 to 545
Restriction enzyme = Bsp1407I number of cut = 1 cut site = 124
recognition sequence = t/gtaca
Table by Enzyme Name
Enzyme No. Positions Recognition
name cuts of sites sequence
Acc113I 1 402 agt/act More in
AcsI 2 338 366 r/aatty More info<
AflIII 1 489 a/crygt More inf
AocI 1 323 cc/tnagg More info<
ApoI 2 338 366 r/aatty More info<
AseI 1 538 at/taat More info<
AsnI 1 538 at/taat More info<
BglII 1 61 a/gatct More info
BsaBI 1 55 gatnn/nnatc More info
Bse21I 1 323 cc/tnagg More inf
Bse8I 1 55 gatnn/nnatc More info
Bsh1365I 1 55 gatnn/nnatc More i
Bsp1407I 1 124 t/gtaca More i
BspLU11I 1 489 a/catgt More i
BsrBRI 1 55 gatnn/nnatc More inf
