-use intrasubtype consensus sequences from 3. as a template in probe designing procedure.
-the probe must be able to hybridized with the PCR product specifically
probe B = >GTAACATTAG TAGAACAAAA TGGAATAACA
probe
E => ACCAGACCCT CCAACAATAC AAGAACAAGT
>Bcon:
-------AAAGCCATGTGTAAAATTAACCCCACTCTGTGTTACTTTAAATTGCATGATA-
--GAATGTACTAAA---------------------TTTACATATAGTAGGGAAATAGGGA
GAAATAAAAAACTGCTCTTTCAATATCACCACAAGCATAAGAGATAAGTGCAGAAAGAAT
ATGCACTTTTTATAAACTTGATGTAGTACCAATAGATAATGAT---AATACAGCTATAGT
TGATAAGTTGTAACACCTCAGTCATTACACAGGCCTGTCCAAAGGTATCCTTTGAGCCAA
TTCCCATACATTATTGTGCCCCGGCTGGTTTTGCGATTCTAAAGTGTAATGATAAGAAGT
TCAATGGAAAGGACCATGTAAAAATGTCAGCACAGTACAATGTACACATGGAATTAGGCC
AGTAGTATCAACTCAACTGCTGTTAAATGGCAGTCTAGCAGAAGAAGAGGTAGTAATTAG
ATCTGCAATTTCACAGACAATCTAAAACCATAATAGTACAGCTGAAGAATCTGTAGAAAT
TAATTGTACAAGACCCAACAACAATACAAGAAAAGTATACATATAGGACCAGGGAGAGCA
TTTATGCAACAGGAGAAATATAGGAGATATAAGACAAGCACATTGTAACATTAGTAGAAC
AAAATGGAATAACACTTTAAAAGATAGTTAAAAATTAAAGAACAATTTAA----------
--ATAAAACAATAGTCTTTAATCATCCTCAGGAGGGGACCCAGAAATTGTAATGCACAGT
TTTAATTGTGGAGGGGAATTTTTCTACTGTAATCAACACAACTGTTTAATAGTACTTGGA
ATACTTGGAATATACTAGGGAAGAGCAATATCACACTCCCATGCAGAATAAAACAAATTA
TAAACATGTGGCAGGAAGTAGGAAAAGCAATGTATGCCCCTCCCATCAGGGACAAATTAG
ATGTTCATCAAATATTACAGGGCTGCTATTAACAAGAGATGGTGGTAATAAACTGA---G
AGCAACCA---CCGAGACTTCAGACCGGAGGAGGAGATATGAGGGACAATTGGAGAAGTG
AATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGGCAA
AGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGT---ACTAGGAGCTTGTTCCTT-----
--------
>Econ
GCCAGTGGTATCAACTCAATTGCTGTTAAATGGCAGTCTAGCAGAAGAAGAGATAATAAT
CAGATCTGAAAATCTCACAAACAATGCCAAAACCATAATAGTGCACCTTAATAAATCTGT
AGAAATCAATTGTACCAGACCCTCCAACAATACAAGAACAAGTATAACTATAGGACCAGG
AC*AGTATTCTATAGAACAGGAGACATAATAGGAGATATAAGAAAAGCATATTGTGAGAT
TAATGGAACAAAATGGAATGAAG*TTTAAAACAGGTAACTGAAAAATTAAAAGAGCACTT
TAA*AATAAGACAATAATCTTTCAACCACCCTCAGGAGGAGATCTAGAAATTACAATGCA
TCATTTTAATTGTAGAGGGGAATTTTTCTATTGCAATACAACA*AACTGTTTAATAATAC
TTGCATAGGAAATGAAACCATGGAGGGGTGTAATGGCACTATCACACTTCCATGCAAGAT
AAAGCAAATTATAAACATGTGGCAGGGAGCAGGACAAGCAATGTATGCTCCTCCCATCAG
TGGAAGAATTAATTGTGTATCAAATATTACAGGAATACTATTGACAAGAGATGGTGGTG-
-----CTAATAATACGATAACGAGACCTTCAGACCTGGAGGAGGAAA-----
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H1 | H2 | H3 | H4 | H5 | H6 | H7 | H8 | H9-H13 |